Recently, scientists from Kolkata based Bose institute has created GlowCas9 which can track gene editing.
About GlowCas9 Protein:
It is a CRISPR protein that lights up while performing gene editing.
It is a bioluminescent version of Cas9 that glows inside cells.
Structure: GlowCas9 is created by fusing Cas9 with a split nano-luciferase enzyme derived from deep-sea shrimp proteins.
Properties of GlowCas9 Protein:
The GlowCas9 is very stable and maintains its structure and activity at higher temperatures compared to the conventional enzyme.
It glows inside cells, allowing for real-time monitoring of CRISPR operations
Working of GlowCas9 Protein:
The split nano-luciferase enzyme pieces reconnect when Cas9 folds correctly, producing light.
This glowing activity allows scientists to monitor CRISPR operations in living cells, tissues, and even plant leaves—without harming them.
Benefits of GlowCas9 Protein
It can also increase the precision of Homology-Directed Repair (HDR).
Homology-Directed Repair (HDR) is a DNA repair process crucial for fixing hereditary mutations that are linked to genetic diseases like sickle cell anaemia, muscular dystrophy and so on.
GlowCas9 pioneers the emerging field of visualizing molecular gene therapy in motion.
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