About Recombinant Proteins:
- These are proteins encoded by recombinant DNA that have been cloned in an expression vector that supports the expression of the gene and translation of messenger RNA.
- Modification of the gene by recombinant DNA technology can lead to the expression of a mutant protein.
- It is a manipulated form of native protein, which is generated in various ways in order to increase the production of proteins, modify gene sequences and manufacture useful commercial products.
- It is created by fusing sequences that may not be normally present in an organism.
- Recombinant proteins, such as vaccine antigens, insulin and monoclonal antibodies, are mass-produced by growing modified bacterial, viral or mammalian cells in large bioreactors. The most widely used organism is the yeast Pichia pastoris (now called Komagataella phaffii).
- Application of Recombinant Proteins
- It is used in biomedical research to understand health and disease.
- It is used in Biotherapeutics.
- It is used to produce protein-based polymers for drug delivery, antibodies and enzymes for disease treatment, protein scaffolds for tissue engineering etc.
What is protein?
- It is the workhorse in biological systems facilitating most of biological processes in a cell, including gene expression, cell growth, proliferation, nutrient uptake, intercellular communication and apoptosis.
- The blue print for protein synthesis is stored in DNA, which serves as a template for highly regulated transcriptional processes to produce messenger RNA (mRNA).
